timsTOF SCP

Expanding the horizons of single cell research

The timsTOF SCP for quantitative single cell biology research with unbiased, deep single-cell 4D-Proteomics™, immunopeptidomics, epiproteomics and PTM analysis to complement scRNA-seq.

High acquisition speed combined with dia-PASEF® sensitivity enables analysis at short liquid chromatography (LC) runs without chromatographic dilution of very low sample amounts.

The challenge of deciphering the proteomes in each single cell – the cell heterogeneity – holds the key to fully understanding its function.

The timsTOF SCP offers a radically improved ion source concept. Combined with parallel accumulation serial fragmentation (PASEF®) acquisition methods, it provides extremely high speed and sensitivity to tackle proteomes of single cells or post translational modifications in a few cells that are morphologically or functionally similar.

Dual-TIMS, CCS-enabled analysis

Trapped ion mobility spectrometry (TIMS) is first and foremost a separation technique in the gas phase. This resolves sample complexity through an added dimension of separation in addition to high performance liquid chromatography (HPLC) and mass spectrometry, increasing peak capacity and confidence in compound characterization.

Equally important, the TIMS device also accumulates and concentrates ions of a given mass and mobility, enabling a unique increase in sensitivity and speed.

A near 100% duty cycle can be achieved with the dual-TIMS technology facilitating accumulation in the front section, while ions in the rear section are sequentially released depending on their mobility.

This process of parallel accumulation serial fragmentation (PASEF®) enables collisional cross section (CCS) analysis.

CCS-enabled analysis opens up many further analytical possibilities, from greater certainty of compound identification to confident library matching and lower false discovery rates (FDRs) in large datasets.